Cholinesterase-inhibitory effect and in silico analysis of alkaloids from bulbs of Hieronymiella species

ORTIZ, JAVIER E.; GARRO, ADRIANA; PIGNI, NATALIA B.; AGÜERO, MARÍA BELÉN; ROITMAN, GERMAN; SLANIS, ALBERTO; ENRIZ, RICARDO D.; FERESIN, GABRIELA E.; BASTIDA, JAUME; TAPIA, ALEJANDRO

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2018 vol. 39 p. 66 - 66

0944-7113

n Argentina, the Amaryllidaceae family (59 species) comprises a wide variety of genera, only a few species have been investigated as a potential source of cholinesterases inhibitors to treat Alzheimer disease (AD).To study the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of the basic dichloromethane extracts (E) from Hieronymiella aurea, H. caletensis, H. clidanthoides, H. marginata, and H. speciosa species, as well as the isolated compounds from these plant extracts.AChE and BChE inhibitory activities were evaluated with the Ellman´s spectrophotometric method. The alkaloids composition from the E was obtained by gas chromatography-mass spectrometry (GC-MS). The E were successively chromatographed on a silica gel column and permeated on Sephadex LH-20 column to afford the main alkaloids identified by means of spectroscopic data. Additionally, an in silico study was carried out.Nine known alkaloids were isolated from the E of five Hieronymiella spe